© 1990 Heron Publishing—Victoria, Canada
Differential responses persist in shoot explants regenerated from callus of two mature black locust trees
K.-H. Han (1), J. M. Davis (2) and D. E. Keathley (2, 3)
1. Department of Forestry, Michigan State University, East Lansing, MI 48824, USA / 2. Department of Biochemistry, SJ-70, University of Washington, Seattle, WA 98195, USA / 3. Author to whom correspondence should be addressed / Received January 16, 1989
Summary
Callus cultures were established from internodal segments of shoot cultures from two mature black locust Robinia pseudoacacia L. trees. Callus of both trees produced shoots on Murashige and Skoog (MS) medium supplemented with 10 μM 6-benzylaminopurine
alone or in combination with 1 μM naphthaleneacetic acid. Regenerated shoots were successfully multiplied on MS medium containing
0.32 μM 6-benzylaminopurine, and produced roots on 0.1 strength MS medium containing 1 μM indole-3-butyric acid. One clone
consistently outperformed the other with respect to shoot proliferation and proportion of shoots that produced roots. This
distinction had previously been observed in shoots produced from bud explants obtained from the mature trees.
