© 1989 Heron Publishing—Victoria, Canada
Evidence of cytokinin bases, ribosides and glucosides in roots of Douglas-fir, Pseudotsuga menziesii
P. Doumas (1), M. Bonnet-Masimbert (1) and J. B. Zaerr (2)
1. INRA, Centre de Recherches d’Orléans, Station d’amelioration des Arbres Forestiers, Ardon 45160 Olivet, France / 2. Department of Forest Science, Oregon State University, Corvallis, OR 97331, USA / Received August 5, 1988
Summary
Concentrations of cytokinin-like compounds were measured in root tips of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) collected in spring. Cytokinin bases and ribosides were estimated by radioimmunoassay after purification
by immunoaffinity (zeatin riboside and isopentenyladenosine antibodies coupled to cellulose gel) and high performance liquid
chromatography. Cytokinin glucosides were similarly analyzed in root extracts treated with β-glucosidase and octadecyl-silica
column chromatography in conjunction with immunoaffinity purification. The identity of predominant cytokinins obtained after
enzymatic degradation was confirmed by gas chromatography–mass spectrometry. Each of the observed cytokinin bases and ribosides
seemed to have a corresponding hexose conjugate form. Total quantity of cytokinin glucosides was twice that of cytokinin bases
and ribosides, and the major compound was isopentenyladenosine hexose conjugate.
