© 1989 Heron Publishing—Victoria, Canada
Shoot culture dynamics of six Populus clones
J. C. Sellmer (1), B. H. McCown (1) and B. E. Haissig (2)
1. Department of Horticulture, University of Wisconsin, Madison, WI 53706, USA / 2. USDA-Forest Service, North Central Forest Experiment Station, P.O. Box 898, Rhinelander, WI 54501, USA / Received November 3, 1988
Summary
Shoot tips of five genotypically diverse Populus clones, P. alba × P. grandidentata ‘Crandon,’ P. nigra ‘Betulifolia’ × P. trichocarpa, P. nigra × P. laurifolia ‘Strathglass,’ P. maximowiczii × P. trichocarpa ‘Androscoggin’ and P. deltoides × P. nigra ‘Eugenei,’ were collected from hardwood cuttings, sterilized,and established in vitro. Stable shoot cultures were obtained
from all clones except P. deltoides × P. nigra ‘Eugenei’. The four poplar clones that formed stable shoot cultures together with a previously established P. tremula ‘Erecta’ clone were placed as two-node explants on either Murashige and Skoog medium or Woody Plant Medium containing benzyladenine
to determine the rate of shoot multiplication, shoot growth and other responses of the clones. All five poplar clones showed
rapid shoot multiplication when cultured in the presence of 0.4–1.0 μM benzyladenine on Murashige and Skoog medium, although
P. tremula ‘Erecta’ produced a greater number of healthy shoots when grown on Woody Plant Medium. Individual shoot growth of all clones
was more vigorous when the medium contained 0–0.1 μM benzyladenine, and 100% of such shoots rooted ex vitro.
