© 1988 Heron Publishing—Victoria, Canada
J 16: An apex protein associated with juvenility of Sequoiadendron giganteum
Marie-Claude Bon (1, 2)
1. Reprint requests to: Marie-Claude Bon, AFOCEL, Domaine de l’Etançon, 77370 Nangis, France / 2. Laboratoire de Phytomorphogenèse de Clermont II, Université de Clermont Ferrand, 4, rue Ledru, 63038 Clermont Ferrand Cedex,
France / Received October 20, 1987
Summary
Shoot apex proteins from 11 seedlings, two juvenile clones and three mature grafted clones of Sequoiadendron giganteum (Lindl.) Buchh. were examined throughout the year by SDS-polyacrylamide gel electrophoresis. A membrane-associated protein
known as J 16, with an approximate molecular weight of 16 kD, was always detected in juvenile tissue, but was absent in tissue
of the mature clones. The J 16 protein was also present in extracts of a mature clone that had been morphologically rejuvenated
by micrografting. Protein extracts from the rejuvenated clone, were separated by electrophoresis and assayed for J 16 using
an immunoblotting procedure with anti-J 16 serum. This test revealed two bands, J 16 and another protein with a lower molecular
weight. The small molecular weight protein was not detected in juvenile clones.
