© 1988 Heron Publishing—Victoria, Canada
Seasonal changes in levels of cytokinin-like compounds from Douglas-fir xylem extrudate
P. Doumas (1, 2) and J. B. Zaerr (1)
1. Department of Forest Science, College of Forestry, Oregon State University, Corvallis, Oregon 97331, USA / 2. Station d’Amélioration des Arbres Forestiers, INRA, Ardon, 45100 Orléans, France / Received February 23, 1987
Summary
High-performance liquid chromatography, immunochromatography, and radioimmunoassay were used to identify cytokinin-like bases
and glycosides in xylem sap of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). Isopentenyladenosine-type (isopentenyladenine and isopentenyladenosine) and zeatin-riboside type (zeatin,
zeatin riboside, and dihydrozeatin riboside) cytokinins were detected during springtime. A glucosyl conjugate of zeatin riboside
was also present in small amounts. Levels of cytokinin-like compounds varied throughout the spring but were generally highest
in late April to early May.
