© 1987 Heron Publishing—Victoria, Canada
In vitro meristem culture of juvenile and mature Sequoiadendron giganteum
O. Monteuuis (1, 2)
1. Association Forêt Cellulose (AFOCEL), Domaine de l’Etançon, 77370 Nangis, France / 2. Laboratoire de Phytomorphogenèse, Université Clermont II, 4, rue Ledru, 63038 Clermont-Ferrand Cédex, France / Received January 29, 1987
Summary
A total of 7000 meristems were used in experiments to investigate the possibility of cloning Sequoiadendron giganteum Buchholz by in vitro meristem culture of juvenile (2-year-old) and mature (100-year-old) ortets. Cultures were initiated
on a low-salt medium containing 0.1 mg l–1
1-naphthaleneacetic acid to stimulate meristematic activity. Benzylamino purine (0.01–0.5 mg l–1) inhibited meristematic activity, whereas gibberellic acid (0.01–0.5 mg l–1) had no effect on meristematic development. The mature ortet showed more specific mineral requirements and a lower capacity
for cloning than the juvenile ortet. Rooted plants were obtained only from the juvenile clone. There was a marked seasonal
effect on meristematic activity, especially for the mature clone, the most active material being obtained during budbreak.
