© 1987 Heron Publishing—Victoria, Canada
Identification and quantitation of indole-3-acetic and abscisic acids in the cambial region of Quercus robur by combined gas chromatography–mass spectrometry
G. Browning and T. A. Wignall
Institute of Horticultural Research, East Malling, Maidstone, Kent ME19 6BJ, UK / Received January 2, 1987
Summary
A technique is described for the rapid purification of indole-3-acetic acid (IAA) and abscisic acid (ABA) from small quantities
of the basal trunk cambial region tissues of 34-year-old trees of Quercus robur L. Mass spectra of IAA and ABA obtained from the purified extracts provide the first reported unequivocal identification
of endogenous IAA and ABA in the cambial region of a hardwood species. By use of the combined gas chromatography–mass spectrometry
technique of multiple ion monitoring, with deuterated analogs as internal standards, it was shown that the levels of IAA in
the cambial region tissues varied from ~1 to ~20 μg g–1 and those of ABA from ~1 to ~5 μg g–1 dry weight during the growing season. The high levels of IAA are similar to those found in the newly differentiating xylem
tissues of softwoods.
