A MADS-box gene of Populus deltoides expressed during flower development and in vegetative organs
Bingyu Zhang (1, 2), Xiaohua Su (1, 2) and Xiangming Zhou (3)
1. Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy
of Forestry, Wan Shou Shan, Beijing, 100091, P.R. China / 2. Corresponding authors (; ) / 3. Tianjin Research Center of Agricultural Biotechnology, Baidi Road 268, Nankai District,Tianjin, 300192, P.R. China / Received September 4, 2007; accepted November 20, 2007; published online April 1, 2008
Summary
A MADS-box gene (PdPI) was isolated from a cDNA library constructed from male flower buds of Populus deltoides Bartr. ex Marsh. According to an analysis of genomic DNA structure and putative protein structure, and a phylogenetic study,
PdPI is an ortholog of the Arabidopsis PI gene. Relative-quantitative real-time polymerase chain reaction analysis showed that PdPI has a broader expression pattern than PI in Arabidopsis. PdPI was strongly expressed in floral buds and roots and weakly expressed in immature xylem, leaves and apical buds of the male
P. deltoides tree. In male inflorescences, PdPI expression was abundant in the perianth and anther, and weak in the peduncle and mature pollen. The large differences in
PdPI expression at various phases of male floral bud development were closely related to the development of flower organs (perianth
and stamen) and pollen. PdPI was also expressed in female inflorescences. Our results suggest that PdPI has multiple functions in the development of P. deltoides.
