Seasonal variation in transcript abundance in cork tissue analyzed by real time RT-PCR
Marçal Soler (1), Olga Serra (1), Marisa Molinas (1), Emili García-Berthou (2), Antònia Caritat (2) and Mercè Figueras (1, 3)
1. Laboratori del Suro, Department of Biology, Universitat de Girona, Campus Montilivi s/n, 17071 Girona, Spain / 2. Department of Environmental Sciences, Universitat de Girona, Campus Montilivi s/n, 17071 Girona, Spain / 3. Corresponding author () / Received August 21, 2007; accepted October 26, 2007; published online March 3, 2008
Summary
The molecular processes underlying cork biosynthesis and differentiation are mostly unknown. Recently, a list of candidate
genes for cork biosynthesis and regulation was made available opening new possibilities for molecular studies in cork oak
(Quercus suber L.). Based on this list, we analyzed the seasonal variation in mRNA abundance in cork tissue of selected genes by real time
reverse-transcriptase polymerase chain reaction (RT-PCR). Relative transcript abundance was evaluated by principal component
analysis and genes were clustered in several functional subgroups. Structural genes of suberin pathways such as CYP86A1, GPAT and HCBT, and regulatory genes of the NAM and WRKY families showed highest transcript accumulation in June, a crucial month for cork development. Other cork structural genes,
such as FAT and F5H, were significantly correlated with temperature and relative humidity. The stress genes HSP17.4 and ANN were strongly positively correlated to temperature, in accord with their protective role.
