Sucrose (JrSUT1) and hexose (JrHT1 and JrHT2) transporters in walnut xylem parenchyma cells: their potential role in early
events of growth resumption
Mélanie Decourteix (1), Georges Alves (1), Marc Bonhomme (2), Mederic Peuch (2), Khaoula Ben Baaziz (1), Nicole Brunel (1), Agnès Guilliot (1), Rémy Rageau (2), Thierry Améglio (2), Gilles Pétel (1) and Soulaïman Sakr (1, 3)
1. UMR 547-PIAF, site des Cézeaux, Université Blaise Pascal, 24 avenue des Landais, 63170 Aubière, France / 2. UMR 547-PIAF, site INRA de Crouelle, 234 avenue du Brézet, 63100 Clermont-Ferrand, France / 3. Corresponding author () / Received July 12, 2006; accepted April 23, 2007; published online December 3, 2007
Summary
In temperate woody species, the vegetative growth period is characterized by active physiological events (e.g., bud break),
which require an adequate supply of soluble sugars imported in the xylem sap stream. One-year-old shoots of walnut (Juglans regia L. cv. ‘Franquette’) trees, which have an acrotonic branching pattern (only apical and distal vegetative buds burst), were
used to study the regulation of xylem sugar transporters in relation to bud break. At the end of April (beginning of bud break),
a higher xylem sap sucrose concentration and a higher active sucrose uptake by xylem parenchyma cells were found in the apical
portion (bearing buds able to burst) than in the basal portion (bearing buds unable to burst) of the sample shoots. At the
same time, xylem parenchyma cells of the apical portion of the shoots exhibited greater amounts of both transcripts and proteins
of JrSUT1 (Juglans regia putative sucrose transporter 1) than those of the basal stem segment. Conversely, no pronounced difference was found for
putative hexose transporters JrHT1 and JrHT2 (Juglans regia hexose transporters 1 and 2). These findings demonstrate the high capacity of bursting vegetative buds to import sucrose.
Immunological analysis revealed that sucrose transporters were localized in all parenchyma cells of the xylem, including vessel-associated
cells, which are highly specialized in nutrient exchange. Taken together, our results indicate that xylem parenchyma sucrose
transporters make a greater contribution than hexose transporters to the imported carbon supply of bursting vegetative buds.
