© 2005 Heron Publishing—Victoria, Canada
Determination of inter- and intra-species genetic relationships among six Eucalyptus species based on inter-simple sequence repeats (ISSR)
T. Balasaravanan (1, 2), P. Chezhian (1), R. Kamalakannan (1), M. Ghosh (1), R. Yasodha (1), M. Varghese (3) and K. Gurumurthi (1)
1. Plant Biotechnology Division, Institute of Forest Genetics and Tree Breeding, Forest Campus, R.S. Puram, Coimbatore - 641
002, Tamil Nadu, India / 2. Corresponding author (balsarvan@rediffmail.com) / 3. Genetics and Tree Breeding Division, Institute of Forest Genetics and Tree Breeding, Forest Campus, R.S. Puram, Coimbatore
- 641 002, Tamil Nadu, India / Received September 29, 2004; accepted February 18, 2005; published online August 1, 2005
Summary
Eucalyptus is the most economically important hardwood plantation tree cultivated in tropical and subtropical countries. Inter-simple
sequence repeat (ISSR) markers were used to evaluate genetic relationships within and between individuals of six Eucalyptus species. A total of 583 loci (265 to 1535 bp) were amplified from 149 individuals belonging to the six Eucalyptus species using seven ISSR primers (two to three nucleotide repeats anchored with one or two nucleotides at the 3′ or 5′ region).
The ISSR fragments indicated significant polymorphism and genetic diversity among the individuals. Cluster analysis and principal
component analysis revealed the occurrence of wide genetic diversity among populations of E. tereticornis Sm., E. camaldulensis Dehnh. and E. urophylla S.T. Blake and narrow genetic diversity among populations of E. citriodora Hook. and E. grandis W. Hill ex Maiden. Genetic diversity was high in E. tereticornis Sm. (47.27%) and low in E. citriodora (18.64%). Maximum Nei’s genetic identity (0.897) was observed between E. camaldulensis and E. tereticornis species, whereas maximum genetic diversity (0.286) was found between individuals of E. citriodora and E. grandis.
Keywords:
between species, genetic diversity, within species.
