© 2000 Heron Publishing—Victoria, Canada
Influencing micropropagation and somatic embryogenesis in mature trees by manipulation of phase change, stress and culture
environment
Patrick von Aderkas (1) and Jan M. Bonga (2)
1. Graduate Centre for Forest Biology, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada (pvonader@uvvm.uvic.ca) / 2. Natural Resources Canada, Canadian Forest Service, Atlantic Forestry Centre, P.O. Box 4000, Fredericton, NB E3B 5P7, Canada / Received April 29, 1998
Summary
This review focuses on clonal propagation of mature trees by tissue culture. Most trees have marked phase changes that result
in a decline in their potential for somatic embryogenesis or micropropagation. By altering conditions of the source material
ex vitro, or by changing in vitro conditions encountered by the explant, rejuvenation and increased propagation can sometimes be accomplished. Various methods
of enhancing micropropagation are reviewed, with particular emphasis on manipulations that involve application of osmotic,
temperature or hormonal stress.
Keywords:
conifers, maturation, rejuvenation, tissue culture.
