© 1997 Heron Publishing—Victoria, Canada
Tissue culture responses of explants taken from branch sources with different degrees of juvenility in mature black locust
(Robinia pseudoacacia) trees
Kyung-Hwan Han (1, 2), Dong-Ill Shin (3) and Daniel E. Keathley (4)
1. Department of Forest Science, Oregon State University, Corvallis, OR 97331, USA / 2. Kumho Life and Environmental Science Laboratories, 572 Ssangamdong, Kwangsangu, Kwangju, Korea / 3. Department of Plant Breeding, College of Natural Resources, Hyusung Catholic University, Hayang, Kyungsan-gun, Kyungpook,
Korea / 4. Department of Forestry, Michigan State University, East Lansing, MI 48824, USA / Received September 9, 1996
Summary
We studied the in vitro responses of cambial tissue and dormant vegetative buds obtained from top and epicormic branches of
three mature black locust (Robinia pseudoacacia L.) trees. Cambial tissues isolated from epicormic branches produced more callus than cambial tissues isolated from top branches,
whereas in vitro shoot cultures derived from buds excised from top branches grew faster than those derived from buds excised
from epicormic branches. There were no significant differences between the two branch sources in in vitro bud break or shoot
multiplication from bud explants or cambial-derived callus tissue, respectively. Furthermore, the top branches, generally
considered to be the most mature in a tree, were not recalcitrant in terms of morphogenic capacity compared to epicormic branches.
Keywords:
black locust, cambial tissue culture, juvenility/maturity, shoot regeneration.
