© 1996 Heron Publishing—Victoria, Canada
Morphogenesis in leaf and single-cell cultures of mature Juniperus oxycedrus
M. P. Gomez and J. Segura
Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100-Burjasot,
Valencia, Spain / Received July 20, 1995
Summary
Single cells were mechanically isolated from leaf-derived callus of mature Juniperus oxycedrus L. These cells divided and gave rise to callus when plated on medium containing growth regulators. Best plating efficiency
was obtained on a modified Schenk and Hildebrandt medium supplemented with 0.6 µM 2,4-dichlorophenoxyacetic acid and 100 mg
l–1 casein hydrolyzate. Although single-cell-derived callus showed poor morphogenic potential, both adventitious shoots and embryogenic
tissues differentiated from the callus. We also achieved induction of somatic embryogenesis in leaf explants of mature J. oxycedrus trees cultured in the presence of 6.0 or 10.0 µM 2,4-dichlorophenoxyacetic acid or picloram. Frequency of embryogenic callus
ranged from 6 to 18%; however, under the culture conditions tested, isolated embryos failed to develop into plants.
Keywords:
adventitious budding, mature explants, somatic embryogenesis.
