© 1996 Heron Publishing—Victoria, Canada
Microculture of western white pine (Pinus monticola) by induction of shoots on bud explants from 1- to 7-year-old trees
Martin S. Lapp, Jana Malinek and Maxine Coffey
Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada, S7N 0W9 / Received June 21, 1995
Summary
We developed a protocol for the production of shoots from bud explants from 1- to 7-year-old trees of western white pine (Pinus monticola Dougl.). The best explant was a 2-mm-thick cross-sectional slice of the early winter bud. Genotype of the donor tree was
a significant factor affecting shoot production, but more than 80% of the genotypes tested produced shoots. Of the media tested,
bud slices from 1- to 3-year-old trees grew best in Litvay’s medium containing N6-benzyladenine in the range of 1 to 30 µM, whereas bud slices from older trees grew best in Gupta and Durzan’s DCR medium
with zeatin riboside. Up to 400 shoots more than 3 mm in height were obtained from 100 bud-slice explants taken from 7-year-old
western white pine trees.
Keywords:
clonal propagation, N6-benzyladenine, organogenesis, shoot induction, zeatin riboside.
