© 1995 Heron Publishing—Victoria, Canada
Micropropagation of Elaeagnus angustifolia from mature trees
J. M. Iriondo, M. de la Iglesia and C. Pérez
Departamento Biología Vegetal, E.T.S. Ingenieros Agrónomos, Universidad Politécnica de Madrid, E-28040 Madrid, Spain / Received November 16, 1994
Summary
Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 µM N6-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional
multiple shoots were obtained. Rooting of the in vitro propagated shoots was achieved on full strength MS medium or on MS
supplemented with 2.46 µM indole-3-butyric acid. Regenerated plantlets were acclimatized and successfully transplanted to
soil.
Keywords:
Elaeagnaceae, in vitro culture, mature explants, nodal explants, oleaster, rooting.
