© 1995 Heron Publishing—Victoria, Canada
Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees
M. P. Gómez and J. Segura
Department of Plant Biology, Faculty of Pharmacy, University of Valencia, Avinguda Vicent Andrés Estellés s/n, 46100-Burjasot
(Valencia), Spain / Received October 17, 1994
Summary
We developed procedures for the micropropagation of Juniperus oxycedrus L. using shoot apices or nodal segments from mature plants. Of the media and explants examined, best culture establishment
was obtained with shoot apices cultured on modified Schenk and Hildebrandt medium (SH medium) without growth regulators; however,
shoot multiplication was only achieved when shoot apices isolated from shoots grown on SH medium without growth regulators
were subcultured on SH medium containing 0.5 µM benzyladenine. None of the auxins and methods tested for root induction provided
satisfactory results.
Keywords:
auxins, benzyladenine, cytokinins, growth regulators, indoleacetic acid, indolebutyric acid, kinetin, naphthaleneacetic acid,
tissue culture.
