© 1994 Heron Publishing—Victoria, Canada
Differentiation of poplar and willow clones using RAPD fingerprints
D. Lin, M. Hubbes and L. Zsuffa
Faculty of Forestry, University of Toronto, Toronto, Ontario M5S 3B3, Canada / Received July 14, 1993
Summary
The technique of random amplified polymorphic DNA (RAPD) fingerprinting was used to differentiate species and identify clones
of 15 poplar and 15 willow clones (ramets of three poplar clones were also included to verify the stability of the results).
Four random DNA primers (Deca-11, Chl-1, Chl-4 and Chl-10) and an M13 universal primer were used to determine DNA polymorphism
among the clones. Based on the DNA banding pattern obtained with the four DNA primers by polymerase chain reaction (PCR) amplification
we conclude that RAPD fingerprinting with properly selected primers can be used
for clonal and species differentiation of poplar and willow. The technique is simple, accurate and inexpensive.
Keywords:
DNA polymorphism, DNA primers, PCR amplification, random amplified polymorphic DNA fingerprinting.
