© 1994 Heron Publishing—Victoria, Canada
Heat-shock response of Pinus and Picea seedlings
David J. Gifford (1) and Edith Taleisnik (2)
1. Botany Department, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada / 2. Instituto de Fitovirologia, INTA, Arturo M. Bas 276, 5000 Córdoba, Argentina / Received July 15, 1993
Summary
The effect of a short period of elevated temperature, or heat shock, on protein synthesis was investigated in 2-day-old seedlings
of jack pine (Pinus banksiana Lamb.), loblolly pine (P. taeda L.), lodgepole pine (P. contorta Dougl.), black spruce (Picea mariana (Mill.) BSP), and white spruce (P. glauca (Moench) Voss.). In all species, heat shock led to increased [35S]methionine incorporation into heat-shock proteins (hsp’s) with molecular masses of 83 and 72 kDA. Heat shock also induced
synthesis of several low molecular weight proteins that were absent from control seedlings. The low molecular weight hsp’s
produced by pine seedlings had molecular masses of 27, 24.6, 20.5 and 17.5 kDa, whereas those produced by spruce seedlings
had molecular masses of 27.2, 19.8, 18.4, 17.2 and 16.0 kDa. All of the low molecular weight hsp’s showed isoelectric variants.
Heat shock led to increased [35S]methionine incorporation into a group of low molecular weight hsp’s that were also present in control seedlings.
Keywords:
black spruce, constitutive, inducible, jack pine, loblolly pine, lodgepole pine, seedling, temperature, white spruce.
