© 1992 Heron Publishing—Victoria, Canada
Localization and quantitative determination of catechins and proanthocyanidins in the phloem of elm and cherry
W. Feucht, D. Treutter and E. Christ
Technical University of Munich, Faculty of Agriculture and Horticulture, D-8050 Freising-Weihenstephan, Germany / Received July 1, 1991
Summary
The localization of catechins and proanthocyanidins in the phloem of elm (Ulmus minor Mill.) and cherry (Prunus avium L.) was determined histochemically by use of 4-dimethylaminocinnamaldehyde (DMACA) reagent. The two tree species showed a
characteristic distribution of these phenols, which were most abundant in the phloem rays of cherry, but were largely confined
to upright parenchyma cells of elm. Quantitative determination and qualitative separation of catechins and proanthocyanidins
were performed using HPLC-CRD (chemical reaction detection), with 4-dimethylaminocinnamaldehyde as the staining reagent. Up
to 14 different catechins and proanthocyanidins were found in both species.
